My Lamineli Sample Buffer Turned Green

One part of regular swimming pool maintenance is chemically shocking a swimming pool.

My lamineli sample buffer turned green. Is it still usuable. Dilute sample 2x laemmli sample buffer. Load on sds page and run. Reference laemmli uk 1970.

Denature proteins by heating samples for 10 minutes at 95 c. Pdb rec084533 2015 cold spring harbor laboratory press full text. 4x laemmli sample buffer. If you needed to add an overdub while mixing a session with lots of tracks and plug ins making the buffer smaller could result in glitching.

Do a check and take a sample to your pool shop. Glycerol sds dtt bpblue water after keeping the 2x in 1 5 tubes for few weeks at 4 degrees i can see the color has changed to dark green and precipitaes at the bottom. Heath samples for 10 minutes at 95 c. Swimming pool owners may find their pool water turns green after they shock it.

For best results do not store sample buffer with 2 mercaptoethanol. Dilute 1 part sample with 1 part 2x laemmli sample buffer. Dilute the 10x loading buffer 1 9 in your sample. Dilute the 4x loading buffer 1 3 in your sample.

Buffers are solutions that resist a change in ph on dilution or on addition of small amounts of acids or alkali. The spot bands in your western are probably caused by incorrect salt content in the sample but could also be from the gels being a bit degraded i ve seen it before with nupage. When swimming pool water turns green after being shocked it is generally because there are metal particles in the water. 30 ml premixed protein sample buffer for peptide and small protein sds page contains 200 mm tris hcl ph 6 8 40 glycerol 2 sds 0 04 coomassie blue g 250.

Dilute β mercaptoethanol 1 19 in your sample i e. More sample buffer can be added if necessary. Sds sample buffer loading turns green with precipitate strange apr 11 2011 dear all here is the recipe for my loading buffer for western 6x. Depending on the size of your pool add liquid chlorine and leave the pump running for 24 hours.

Dilute 3 parts sample with 1 part 4x laemmli sample buffer. You can add 5 litres of liquid chlorine to a 35 000 litre pool and still swim without your hair going green but you may need to add a buffer and stabiliser. The green colour is definitely a ph change i suspect that either your tris buffer is incorrectly prepared or there is something wrong with the lysis buffer. Doi 10 1101 pdb rec084533 cold spring harb protoc 2015.